ZOOLOGY FOR BSC STUDENTS - SEM III [NEP-UP]

This textbook has been designed to meet the needs of B.Sc. Third Semester students of Zoology as per Common Minimum Syllabus prescribed for all Uttar Pradesh State Universities and Colleges under the recommended National Education Policy 2020. It comprehensively covers two papers, namely, theory paper on Molecular Biology, Bioinstrumentation and Biotechniques and practical paper on Bioinstrumentation and Molecular Biology Lab. The Molecular Biology part of the book emphasizes the fundamental features of various aspects of DNA, RNA, and protein structure, function, and expression. The regulation of Gene expression in Prokaryotes and Eukaryotes is presented in a very lucid and comprehensive way. · More than 250 well-labelled diagrams have been provided for easy understanding of the concepts · Boxed items throughout the text providing information on intricate terms or topics · Engaging chapter-end exercises to enhance and strengthen learning PAPER I: Molecular Biology, Bioinstrumentation & Biotechniques 1: Definition, History and Prologue 2: Organisms Used in Molecular Biology 3: Fine Structure of Gene 4: Transcription 5: Messenger RNA 6: Ribosomal RNA (rRNA) and Transfer RNA (tRNA) 7: Genetic Code 8: Process of Translation 9: Post-Translational Processing of Proteins 10: Regulation of Gene Expression in Bacteria (Prokaryotes) and Viruses 11: Regulation of Gene Expression in Eukaryotes 12: Regulatory RNAs: Riboswitches, RNA Interference (RNAi), siRNAs and miRNAs 13: Principle and Types of Microscopes 14: Centrifugation and Chromatography 15: Biochemical Techniques: Measurement of pH 16: Colorimetry and Spectrophotometry 17: Measurement, Applications and Safety Measures of Radio Tracer Techniques 18: Molecular Techniques: Electrophoresis 19: Molecular Techniques 20: Polymerase Chain Reaction (PCR) 21: Proteomics: Detection of Proteins, PAGE, ELISA and Western Blotting PAPER II: Bioinstrumentation & Molecular Biology Lab 1: Microscopes 2: To Prepare Solutions and Buffers 3: Experiments in Spectrophotometry and Chromatography • Viva-Voce Questions